martinlukasesd: 2012

Monday, December 31, 2012

FS: iPhone 4s Verizon 16GB Black

OK I had to start another thread because I think I found a laptop to buy so the phone is back up for cash sale but until I purchase the laptop I am still open to hear laptop trade offers. If you have a laptop and want to trade please search for my other post in this section to see what I am looking for.

The iPhone is very clean with no scratches or scuffs and is currently activated and working on Verizon so it has a clean ESN
The iPhone is Black 16GB comes in original box with all accessories including charger, iPhone cable, headphones and manuals
I am throwing in 2 New White rubber cases and 2 New black rubber cases and 1 Otter Box Defender used without the belt clip

I am currently looking to break even on the laptop I am looking to buy so current asking price is $225 + S&H

I don't want to mess with unlocking, jail breaking or anything else, I will leave that up to the buyer

Heatware: mypartsguy I have an 800+ rating with Paypal and my account is Verified Premier

Source: http://hardforum.com/showthread.php?t=1736945&goto=newpost

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Boston College coach York sets NCAA wins record

Jerry York's place among the greatest coaches in college hockey history has long since been sealed, but there are now no others who have experienced more success.

Boston College defeated Alabama-Huntsville 5-2 on Saturday night in the opening round of the Mariucci Classic in Minneapolis, and the win is No. 925 in York's storied career. It pushes him past Ron Mason for the most in NCAA hockey history, after York had tied the former Lake Superior State, Bowling Green and Michigan State coach in Boston College's 5-2 win Dec. 1 against rival Boston University. York's team had played only one game in the previous 28 days -- a 3-3 tie at Providence on Dec. 7.

York began his coaching career at Clarkson in 1972-73 before moving to Bowling Green and replacing Mason in 1979-80. He won four conference titles with the Falcons, and his first of five national titles in 1984, before joining Boston College for the 1994-95 season.

He actually finished below .500 in his first three seasons with Boston College, but York's team reached the championship game in his fourth year and has been one of the most consistent powerhouses in the sport since. York has reached the Frozen Four 10 times in the past 15 seasons, winning four championships.

Boston College is the defending champion, and York's team has won three of the past five titles. Given that the Eagles improved to 13-2-1 with the victory against Alabama-Huntsville and are ranked No. 1 in both the USCHO.com and USA Today/USA Hockey Magazine polls, York could be in a position to add a sixth title to his resume in April.

Source: http://www.nhl.com/ice/news.htm?id=648522

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Who might fill the NFL coaching openings

When NFL coaching jobs open, the names Jon Gruden, Bill Cowher and Tony Dungy immediately surface as potential candidates.

Much more likely than any of those Super Bowl winners returning to the sideline for 2013 would be the hirings of more obscure assistant coaches such as Mike Zimmer, Mike McCoy and Gus Bradley.

And Jon Gruden's brother, Jay.

Sure, some of the best-known coaches, including Andy Reid and Lovie Smith, who lost their jobs Monday, will be in the mix. So might college coaches Chip Kelly of Oregon and Bill O'Brien of Penn State.

Maybe even Nick Saban, although leaving Alabama for the NFL is a long shot.

___

Online: http://pro32.ap.org/poll and http://twitter.com/AP_NFL

Source: http://news.yahoo.com/might-fill-nfl-coaching-openings-180445697--nfl.html

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College football bowls: 5 games on the last Saturday ever

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2012 Armed Forces Bowl results: Rice rallies to beat Air Force, 33-14

Behind a strong defensive effort and a breakout performance by backup quarterback Driphus Jackson, the Rice Owls earned a two-touchdown victory over the Air Force Falcons, who gained a season-low 214 yards of total offense.

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Pinstripe Bowl? More like Snowstripe Bowl, amirite?

It's snowing at Yankee Stadium, and everything is wonderful.

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Finding Your Voice: Speak Up and Change Your World in 2013 ...

Happy New Year! Over the past five years I have touched on numerous topics that are related to healing the workplace. This blog emerged out of my own experience working in an organization that needed healing on many levels.

One of the challenges that all of us face, at one time or another, is to find ways to initiate positive change so that everyone is treated with respect and treated fairly.

We all have the ability to speak up and bear witness to the injustices that we see, but it is not easy. Speaking up in this way requires that we ?find out voice? and find ways to use it. We all have a voice?some of us are perhaps more quieter than others.

Some?of us are?fortunate enough to live in Canada, which is a democratic country. Living in Canada makes it possible for us to speak up and voice our opinions and offer suggestions. It also means that we can challenge the status quo and take positive action.

Paradoxically finding one?s voice begins with listening. We must listen to our intentions, to our values, to our experience and to our intuition. Perhaps most importantly, we have to listen to other people.

Reflections on finding my voice:

Finding my voice whether I?m writing or speaking has been challenging for me.
Finding my voice means speaking up and defending what I believe in.
Finding my voice means having the courage to speak out against injustices.
Finding my voice means letting who I am emerge on the pages that I am writing.
Finding my voice means being authentic?it means living my values by demonstrating them in my daily actions.
Finding my voice means pushing myself out of my comfort zone.
Finding my voice means not talking so others can talk.
Finding my voice is one of the hardest things I?ve ever had to do.

If we want to change our workplaces, our communities and our world we need to find ?our collective voice. But it really starts with each one of us?one voice at a time.

Happy New Year

Lesley Taylor

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Source: http://healingtheworkplace.wordpress.com/2012/12/30/finding-your-voice-speak-up-and-change-your-world-in-2013/

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Saturday, December 29, 2012

Wall St Week Ahead - Cliff may be a fear, but debt ceiling much scarier

REUTERS - Investors fearing a stock market plunge - if the United States tumbles off the "fiscal cliff" next week - may want to relax.

But they should be scared if a few weeks later, Washington fails to reach a deal to increase the nation's debt ceiling because that raises the threat of a default, another credit downgrade and a panic in the financial markets.

Market strategists say that while falling off the cliff for any lengthy period - which would lead to automatic tax hikes and stiff cuts in government spending - would badly hurt both consumer and business confidence, it would take some time for the U.S. economy to slide into recession. In the meantime, there would be plenty of chances for lawmakers to make amends by reversing some of the effects.

That has been reflected in a U.S. stock market that has still not shown signs of melting down. Instead, it has drifted lower and become more volatile.

In some ways, that has let Washington off the hook. In the past, a plunge in stock prices forced the hand of Congress, such as in the middle of the financial crisis in 2008.

"If this thing continues for a bit longer and the result is you get a U.S. debt downgrade ... the risk is not that you lose two-and-a-half percent, the risk is that you lose ten and a half," said Jonathan Golub, chief U.S. equity strategist at UBS Equity Research, in New York.

U.S. Treasury Secretary Tim Geithner said this week that the United States will technically reach its debt limit at the end of the year.

INVESTORS WARY OF JANUARY

The White House has said it will not negotiate the debt ceiling as in 2011, when the fight over what was once a procedural matter preceded the first-ever downgrade of the U.S. credit rating. But it may be forced into such a battle again. A repeat of that war is most worrisome for markets.

Markets posted several days of sharp losses in the period surrounding the debt ceiling fight in 2011. Even after a bill to increase the ceiling passed, stocks plunged in what was seen as a vote of "no confidence" in Washington's ability to function, considering how close lawmakers came to a default.

Credit ratings agency Standard & Poor's lowered the U.S. sovereign rating to double-A-plus, citing Washington's legislative problems as one reason for the downgrade from triple-A status. The benchmark S&P 500 dropped 16 percent in a four-week period ending August 21, 2011.

"I think there will be a tremendous fight between Democrats and Republicans about the debt ceiling," said Jon Najarian, a co-founder of online brokerage TradeMonster.com, in Chicago.

"I think that is the biggest risk to the downside in January for the market and the U.S. economy."

There are some signs in the options market that investors are starting to eye the January period with more wariness. The CBOE Volatility Index <.vix>, or the VIX, the market's preferred indicator of anxiety, has remained at relatively low levels throughout this process, though on Thursday it edged above 20 for the first time since July.

More notable is the action in VIX futures markets, which shows a sharper increase in expected volatility in January than in later-dated contracts. January VIX futures are up nearly 23 percent in the last seven trading days, compared with a 13 percent increase in March futures and an 8 percent increase in May futures. That's a sign of increasing near-term worry among market participants.

The CBOE Volatility Index closed on Friday at 22.72, gaining nearly 17 percent to end at its highest level since June as details emerged of a meeting on Friday afternoon of President Barack Obama with Senate and House leaders from both parties where the president offered proposals similar to those already rejected by Republicans. Stocks slid in late trading and equity futures continued that slide after cash markets closed.

"I was stunned Obama didn't have another plan, and that's absolutely why we sold off," said Mike Shea, a managing partner and trader at Direct Access Partners LLC, in New York.

Obama offered hope for a last-minute agreement to avoid the fiscal cliff after a meeting with congressional leaders, although he scolded Congress for leaving the problem unresolved until the 11th hour.

"The hour for immediate action is here," he told reporters at a White House briefing. "I'm modestly optimistic that an agreement can be achieved."

The U.S. House of Representatives is set to convene on Sunday and continue working through the New Year's Day holiday. Obama has proposed maintaining current tax rates for all but the highest earners.

Consumers don't appear at all traumatized by the fiscal cliff talks, as yet. Helping to bolster consumer confidence has been a continued recovery in the housing market and growth in the labor market, albeit slow.

The latest take on employment will be out next Friday, when the U.S. Labor Department's non-farm payrolls report is expected to show jobs growth of 145,000 for December, in line with recent growth.

Consumers will see their paychecks affected if lawmakers cannot broker a deal and tax rates rise, but the effect on spending is likely to be gradual.

PLAYING DEFENSE

Options strategists have noted an increase in positions to guard against weakness in defense stocks such as General Dynamics because those stocks would be affected by spending cuts set for that sector. Notably, though, the PHLX Defense Index is less than 1 percent away from an all-time high reached on December 20.

This underscores the view taken by most investors and strategists: One way or another, Washington will come to an agreement to offset some effects of the cliff. The result will not be entirely satisfying, but it will be enough to satisfy investors.

"Expectations are pretty low at this point, and yet the equity market hasn't reacted," said Carmine Grigoli, chief U.S. investment strategist at Mizuho Securities USA, in New York. "You're not going to see the markets react to anything with more than a 5 (percent) to 7 percent correction."

Save for a brief 3.6 percent drop in equity futures late on Thursday evening last week after House Speaker John Boehner had to cancel a scheduled vote on a tax-hike bill due to lack of Republican support, markets have not shown the same kind of volatility as in 2008 or 2011.

A gradual decline remains possible, Golub said, if business and consumer confidence continues to take a hit on the back of fiscal cliff worries. The Conference Board's measure of consumer confidence fell sharply in December, a drop blamed in part on the fiscal issues.

"If Congress came out and said that everything is off the table, yeah, that would be a short-term shock to the market, but that's not likely," said Richard Weiss, a Mountain View, California-based senior money manager at American Century Investments.

"Things will be resolved, just maybe not on a good time table. All else being equal, we see any further decline as a buying opportunity."

(Reporting by Edward Krudy and Ryan Vlastelica in New York and Doris Frankel in Chicago; Writing by David Gaffen; Editing by Martin Howell, Steve Orlofsky and Jan Paschal)

Source: http://news.yahoo.com/wall-st-week-ahead-cliff-may-fear-debt-235250243--finance.html

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Putin signs anti-US adoptions bill

Russian President Vladimir Putin speaks at the final Cabinet meeting of the year in the government headquarters in Moscow, Thursday, Dec. 27, 2012. (AP Photo/RIA-Novosti, Dmitry Astakhov, Government Press Service)

MOSCOW (AP) ? President Vladimir Putin on Friday signed a law banning Americans from adopting Russian children, abruptly terminating the prospects for more than 50 youngsters preparing to join new families and sparking critics to liken him to King Herod.

The move is part of a harsh response to a U.S. law targeting Russians deemed to be human rights violators. Although some top Russian officials including the foreign minister openly opposed the bill, Putin signed it less than 24 hours after receiving it from Parliament, where it passed both houses overwhelmingly.

The law also calls for the closure of non-governmental organizations receiving American funding if their activities are classified as political ? a broad definition many fear could be used to close any NGO that offends the Kremlin.

The law takes effect Jan. 1, the Kremlin said. Children's rights ombudsman Pavel Astakhov said 52 children who were in the pipeline for U.S. adoption would remain in Russia.

The ban is in response to a measure signed into law by President Barack Obama this month that calls for sanctions against Russians assessed to be human rights violators.

That stems from the case of Sergei Magnitsky, a Russian lawyer who was arrested after accusing officials of a $230 million tax fraud. He was repeatedly denied medical treatment and died in jail in 2009. Russian rights groups claimed he was severely beaten.

A prison doctor who was the only official charged in the case was acquitted by a Moscow court on Friday. Although there was no demonstrable connection to Putin's signing the law a few hours later, the timing underlines what critics say is Russia's refusal to responsibly pursue the case.

The adoption ban has angered both Americans and Russians who argue it victimizes children to make a political point, cutting off a route out of frequently dismal orphanages for thousands.

"The king is Herod," popular writer Oleg Shargunov said on his Twitter account, referring to the Roman-appointed king of Judea at the time of Jesus Christ's birth, who the Bible says ordered the massacre of Jewish children to avoid being supplanted by a prophesied newborn king of the Jews.

A painting depicting the massacre and captioned "an appropriate response to the Magnitsky act" spread widely on the Internet. The phrase echoed Putin's characterization of the ban while it was under consideration.

U.S. State Department spokesman Patrick Ventrell expressed regret over Putin's signing the law and urged Russia to "allow those children who have already met and bonded with their future parents to finish the necessary legal procedures so that they can join their families."

Vladimir Lukin, head of the Russian Human Rights Commission and a former ambassador to Washington, said he would challenge the law in the Constitutional Court.

The U.S. law galvanized Russian resentment of the United States, which Putin has claimed funded and encouraged the wave of massive anti-government protests that arose last winter.

The Parliament initially considered a relatively similar retaliatory measure, but amendments have expanded it far beyond a tit-for-tat response.

UNICEF estimates that there are about 740,000 children not in parental custody in Russia while about 18,000 Russians are on the waiting list to adopt a child. The U.S. is the biggest destination for adopted Russian children ? more than 60,000 of them have been taken in by Americans over the past two decades.

Russians historically have been less enthusiastic about adopting children than most Western cultures. Putin, along with signing the adoption ban, on Friday issued an order for the government to develop a program to provide more support for adopted children.

Lev Ponomarev, one of Russia's most prominent human rights activists, hinted at that reluctance when he said Parliament members who voted for the bill should take custody of the children who were about to be adopted.

"The moral responsibility lies on them," he told Interfax. "But I don't think that even one child will be taken to be brought up by deputies of the Duma."

Many Russians have been distressed for years by reports of Russian children dying or suffering abuse at the hands of their American adoptive parents. The new Russian law was dubbed the "Dima Yakovlev Bill" after a toddler who died in 2008 when his American adoptive father left him in a car in broiling heat for hours.

In that case, the father was found not guilty of involuntary manslaughter and Russia has complained of acquittals or light sentences in other such cases.

The Investigative Committee, Russia's top investigative body, on Friday complained that its attempts to have the acquittals overturned or reconsidered had been ignored by the United States. Under U.S. law, acquittals are final except in rare cases.

Russians also bristled at how the widespread adoptions appeared to show them as hardhearted or too poor to take care of orphans. Astakhov, the children's ombudsman, charged that well-heeled Americans often got priority over Russians who wanted to adopt.

A few lawmakers even claimed that some Russian children were adopted by Americans only to be used for organ transplants or become sex toys or cannon fodder for the U.S. Army. A spokesman for Russia's dominant Orthodox Church said that children adopted by foreigners and raised outside the church will not enter God's kingdom.

___

Mansur Mirovalev and Nataliya Vasilyeva in Moscow contributed to this story.

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/cae69a7523db45408eeb2b3a98c0c9c5/Article_2012-12-28-Russia-US-Adoption/id-72bcfefa3c2e44ee9e4b85ed0c820156

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56% of female university students get drunk in record time, Spanish study suggests

Dec. 28, 2012 ? Researchers from the HealthyFit group at the University of Vigo have studied university students' lifestyles; their analysis, which includes alcohol and illegal drug consumption habits, sport and food, concludes that most students indulge in unhealthy behaviour. One of the main results of the study is the high consumption of alcohol.

"The amount drunk per unit of time is higher among women. In other words, even though male students drink more often, females do so more intensively in shorter periods of time, which is known as binge drinking," explained Jos? M? Cancela Carral, co-author of the study published by the Journal of Environmental Research and Public Health.

Researchers randomly selected 985 students from different degree courses and in different years at the University of Vigo.

Of the females interviewed, 51.2% lead a sedentary lifestyle, while the percentage in males is 41.7%. When analysing students who maintain an appropriate level of physical activity, 38.6% of males do physical exercise, as opposed to only 20.9% of women.

"We were also surprised by the high consumption of illegal drugs among university students -- 44.9% of men and 30.9% of women -- which we understand could lead to significant future health problems, mainly related to the nervous system," underlined the researcher.

Anomalous attitudes to food were more evident among women (16.6%), although also present among men (8.8%). "However, the statistical analysis showed that this parameter depended on the degree the student was studying for," added Cancela. Such attitudes were much more common among those studying degrees related to education (19.2%) than among those studying courses related to health (6.3%).

Health network

Spanish universities set up a Healthy University Network in 2008, a project for healthy living for universities from all over Spain, the Spanish University Rectors' Association, the Ministry of Health, Social Services and Equality, the Ministry of Education, Culture and Sport and some regional public health entities.

In the researcher's opinion, at many universities this network was nothing more than "a simple first step to get on the list and nothing else"; hence transversal content should be implemented in study plans related to food, physical exercise and healthy habits.

"In the light of the results, training and information courses are required in these areas, together with healthy leisure -- not just sports facilities -- to set up university guidance services for a healthy student lifestyle," concludes Cancela.

Genetic predisposition

A recent study published in the PNAS journal argues that the gene called RASGRF2 could be related to a predisposition to getting drunk.

According to scientists, this gene regulates the predisposition to drink excessive amounts of alcohol as it influences mesolimbic dopamine neuron activity, which is one of the ways dopamine is taken from one region of the brain to another.

Gunter Schumann, one of the main authors of the study, explained that even though we should not consider said gene to be the main cause -- as there are many environmental factors and other genes involved -- the study thereof helps to explain why some people are more vulnerable to alcohol than others.

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The above story is reprinted from materials provided by Plataforma SINC, via AlphaGalileo.

Note: Materials may be edited for content and length. For further information, please contact the source cited above.

Journal References:

Ver?nica Varela-Mato, Jos? M. Cancela, Carlos Ayan, Vicente Mart?n, Antonio Molina. Lifestyle and Health among Spanish University Students: Differences by Gender and Academic Discipline. International Journal of Environmental Research and Public Health, 2012; 9 (8): 2728 DOI: 10.3390/ijerph9082728
D. Stacey, A. Bilbao, M. Maroteaux, T. Jia, A. C. Easton, S. Longueville, C. Nymberg, T. Banaschewski, G. J. Barker, C. Buchel, F. Carvalho, P. J. Conrod, S. Desrivieres, M. Fauth-Buhler, A. Fernandez-Medarde, H. Flor, J. Gallinat, H. Garavan, A. L. W. Bokde, A. Heinz, B. Ittermann, M. Lathrop, C. Lawrence, E. Loth, A. Lourdusamy, K. F. Mann, J.-L. Martinot, F. Nees, M. Palkovits, T. Paus, Z. Pausova, M. Rietschel, B. Ruggeri, E. Santos, M. N. Smolka, O. Staehlin, M.-R. Jarvelin, P. Elliott, W. H. Sommer, M. Mameli, C. P. Muller, R. Spanagel, J.-A. Girault, G. Schumann. RASGRF2 regulates alcohol-induced reinforcement by influencing mesolimbic dopamine neuron activity and dopamine release. Proceedings of the National Academy of Sciences, 2012; 109 (51): 21128 DOI: 10.1073/pnas.1211844110

Note: If no author is given, the source is cited instead.

Disclaimer: This article is not intended to provide medical advice, diagnosis or treatment. Views expressed here do not necessarily reflect those of ScienceDaily or its staff.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/most_popular/~3/JCkJkf4_4pY/121228084029.htm

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Top 25 Best Xbox 360 Games of 2012 - Video Games Blogger

The Top 25 best Xbox 360 games of 2012 have been revealed! Is your favorite Xbox 360 game part of the list of the greatest the Xbox 360 console had to offer in 2012? The year is almost over, so we?ve compiled this list of the top-quality AAA must-own titles of the year!

Is there a game that didn?t make the list, that you think deserves a spot? Leave a comment and let us know your own list!

Runners-Up:

Call of Duty: Black Ops II, Sine Mora, Tekken Tag Tournament 2, Dust: An Elysian Tail, Minecraft: Xbox 360 Edition, The Pinball Arcade, Sonic & All-Stars Racing Transformed, Pro Evolution Soccer 2013, SSX, and Joe Danger 2: The Movie.

#25 ? Street Fighter X Tekken
#24 ? Persona 4 Arena
#23 ? Darksiders II
#22 ? NHL 13
#21 ? F1 2012
#20 ? Assassin?s Creed III
#19 ? Need for Speed: Most Wanted
#18 ? UFC Undisputed 3
#17 ? Forza Horizon (Xbox 360 Exclusive)
#16 ? Max Payne 3
#15 ? Dance Central 3 (Xbox 360 Kinect Exclusive)
#14 ? Spelunky
#13 ? Halo 4 (Xbox 360 Exclusive)
#12 ? NBA 2K13
#11 ? The Witcher 2: Assassins of Kings (Xbox 360 Version)

#10 ? Dishonored

#9 ? Fez (Xbox 360 Exclusive)

#8 ? Borderlands 2

#7 ? XCOM: Enemy Unknown

#6 ? FIFA Soccer 13

Find out which Xbox 360 game is #1 in 2012 as our countdown continues on the next page?

Continue Reading on: Page 1 Page 2

About the author

By Ferry Groenendijk: He is the founder and editor of Video Games Blogger. He loved gaming from the moment he got a Nintendo with Super Mario Bros. on his 8th birthday. Learn more about him here and connect with him on Twitter, Facebook and at Google+.

Source: http://www.videogamesblogger.com/2012/12/27/top-25-best-xbox-360-games-of-2012.htm

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PFT: Sherman, Sullivan among biggest Pro Bowl snubs

It?s obvious the Giants defense isn?t what it once was.

But if you ask their defensive coordinator why, he makes it clear it?s not the coaching.

?We?ve prepared better than what we?ve played,? Perry Fewell said, via Ralph Vacchiano of the New York Daily News. ?I think if you look at the tape we?re in position to make plays. We haven?t made plays.?

While coaches default into self-preservation mode this time of year, it?s hard to pin the blame for the Giants? precipitous slide on one man.

They were seventh in the league in total defense in 2010, and won a Super Bowl a year ago. This year, they?re 30th in total defense.

But mostly, they?ve been awful when it counted most, allowing 67 points in their last two must-win games, which leaves them only a slim conditional chance at the playoffs heading into the final weekend.

?We?ve been in position,? Fewell said. ?We haven?t made a football play for the last couple of weeks. We?ve been in position to make football plays, but it hasn?t gone our way.?

If there?s a sign that players know Fewell?s right, it?s that there?s no open or indirect grumbling about him yet.

?We won a Super Bowl in this system ? I think that?s the main thing,? linebacker Mathias Kiwanuka said. ?We had the same system that we did last year.?

?There?s a lot of support for Perry,? defensive end Osi Umenyiora added. ?I?ll just say that.?

Of course, the Giants were trending this direction defensively last year, finishing 27th in the league. But their play late in the year was much of the reason they won a title, so that fades from memory. That?s added some insulation for Fewell, and any offseason changes should rightly begin with personnel.

He?s had chances to interview for head coaching jobs in the past (and the Panthers may have made a significant mistake picking Ron Rivera over him), and will likely continue to. But until he gets one of those jobs, he?s left trying to scheme for an aging cast which needs a re-tooling.

Source: http://profootballtalk.nbcsports.com/2012/12/26/sullivan-campbell-sherman-among-biggest-pro-bowl-snubs/related/

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Friday, December 28, 2012

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Source: http://www.lapappalpomodoro.com/2012/12/27/cms-internet-site-sydney-cellular-sydney-natural-seo-seo-firm-sydney-seo-services-sydney/172

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Monday, December 24, 2012

Swedish princess to marry next June

STOCKHOLM (AP) ? Sweden's royal family says Princess Madeleine's wedding will take place on Saturday, June 8, at the Royal Palace chapel in downtown Stockholm.

King Carl XVI Gustaf and Queen Silvia's youngest daughter was engaged to U.S.-British boyfriend Christopher O'Neill, a 38-year-old New York banker, in late October. The 30-year-old Madeleine is fourth in line to the Swedish throne.

The royal family's chief spokesman Bertil Tenert said Sunday that wedding organizers will now start planning details of the nuptials. He added that Madeleine's wedding will be smaller than Crown Princess Victoria's wedding two years ago in Stockholm.

The Swedish royal family has only ceremonial duties, such as supporting charities and promoting Swedish businesses.

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/cae69a7523db45408eeb2b3a98c0c9c5/Article_2012-12-23-Sweden-Princess%20Madeleine/id-c089bc619553470097721cf52060e6d3

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First goat genome sets a good example for facilitating de novo assembly of large genomes

Dec. 23, 2012 ? In a collaborative study published online today in Nature Biotechnology, researchers from Kunming Institute of Zoology, Chinese Academy of Sciences, BGI, and other institutes, have completed the first genome sequence of domestic goat by a robust approach integrated with next-generation sequencing (NGS) and whole-genome mapping (WGM) technologies. The goat genome is the first reference genome for small ruminant animals and may help to advance the understanding of distinct ruminants' genomic features from non-ruminant species. This work also yields a valuable experience for facilitating the de novo assemblies of large, complex genomes in the future.

Goats are recognized as an important member of the world livestock industry, and with many unique biological features. They are an important economic resource in many developing countries around the world, especially in China and India. However, despite their agricultural and biological importance, breeding and genetic studies of goats have been hampered by the lack of a high quality reference genome sequence. The goat genome sequence will be useful for facilitating the identification of SNP markers for marker-assisted breeding, and improving the utility of the goat as a biomedical model and bioreactor.

With the availability of next-generation sequencing (NGS), draft assemblies are easy to generate nowadays. However, to finish a sequence to the chromosome level remains a hard nut to crack. In this study, the results show that a single NGS platform, when combined with whole-genome mapping technology, could produce a finished assembly much faster and with high quality than other currently available mapping strategies such as BACs or FISH. Through this integrated approach, researchers obtained the ~2.66 Gb goat reference genome from a female Yunnan black goat.

Transposable elements (TEs) are major components of mammalian genomes and contribute to gene and/or genome evolution. The TEs in goat genome are similar to those of cattle, and contain large numbers of ruminant-specific repeats, such as SINE-tRNA and SINE-BovA. It is reported that SINE-BovA repeat expanded primarily in the cattle genome. However, in this study, researchers found the SINE-tRNA repeat expanded specifically in the goat genome.

Through constructing a phylogenetic tree among goats, cattle, horses, dogs, opossums and humans, researchers found the goat shared a common ancestor with cattle about 23 million years ago. Further comparison analysis revealed 44 rapidly evolving genes under positive selection, seven of which are immune-related genes and three are pituitary hormone or related genes. The immune-related genes identified also exist in cattle. The findings suggest that the rapid evolution of pituitary hormones may be related to the different features between goat and cattle in milk production, development rates of the fetus and/or hair variation.

The major histocompatibility complex (MHC) plays an important role in the immune system. In this study, the goat MHC was found to be located on chromosome 23 and contains two regions with length of 2.25 Mb and 360 kb, respectively. With the high quality genome assembly, further understanding of the goat MHC will be useful for immunological studies and vaccine development.

One of the distinguishing characteristics of mammals is the protective growth known as hair. It is produced by hair follicles within the skin, which could provide either protection (guard hairs) or insulation (underfur). The two major hair follicles include the primary hair follicle that produces only coat hair in all mammals, and the secondary hair follicle that can produce the cashmere or "fine hair" in certain mammals, including goats and antelopes. Despite a 2,500-year history and the extent of raw cashmere production, people are lack of understanding of the molecular mechanisms of cashmere formation and development.

Researchers conducted transcriptomic analysis on the primary and secondary follicles of a cashmere goat, revealing 51 genes that are differentially expressed between the two types of hair follicles. Keratin and keratin-associated proteins are the main structural proteins of hair fibers, determining the quality of fiber together. In the study, 29 keratin genes and 30 keratin-associated protein genes were detected in both types of follicles. Interestingly, they found two keratin genes and ten keratin-associated protein genes were consistently differentially expressed between primary and secondary hair follicles, suggesting that the keratin-associated protein genes may be more important in determining the structure of cashmere fibers. In addition to the keratin genes and keratin-associated protein genes, researchers also found several enzymes of amino acid biosynthesis, with implications in regulating primary hair growth and hair cycle.

Xun Xu, Deputy Director of BGI, said, "The goat reference genome is an important stepping stone in the molecular breeding of cashmere goats, and will help to advance the comparative studies on ruminants. The transcriptomic analysis on the primary and secondary follicles will open a new way for better improving the quality cashmere wool."

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The above story is reprinted from materials provided by BGI Shenzhen, via EurekAlert!, a service of AAAS.

Note: Materials may be edited for content and length. For further information, please contact the source cited above.

Journal Reference:

Yang Dong, Min Xie, Yu Jiang, Nianqing Xiao, Xiaoyong Du, Wenguang Zhang, Gwenola Tosser-Klopp, Jinhuan Wang, Shuang Yang, Jie Liang, Wenbin Chen, Jing Chen, Peng Zeng, Yong Hou, Chao Bian, Shengkai Pan, Yuxiang Li, Xin Liu, Wenliang Wang, Bertrand Servin, Brian Sayre, Bin Zhu, Deacon Sweeney, Rich Moore, Wenhui Nie, Yongyi Shen, Ruoping Zhao, Guojie Zhang, Jinquan Li, Thomas Faraut, James Womack, Yaping Zhang, James Kijas, Noelle Cockett, Xun Xu, Shuhong Zhao, Jun Wang, Wen Wang. Sequencing and automated whole-genome optical mapping of the genome of a domestic goat (Capra hircus). Nature Biotechnology, 2012; DOI: 10.1038/nbt.2478

Note: If no author is given, the source is cited instead.

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Source: http://feeds.sciencedaily.com/~r/sciencedaily/most_popular/~3/CNn97tXK9DU/121223152629.htm

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